etfA [2019-05-10 14:20:00]

electron transfer flavoprotein (alpha subunit)

Locus: BSU28520

Isoelectric point: 4.65

Molecular weight: 34.39 kDa

Protein length: 325 aa

Gene length: 978 bp

Function: fatty acid degradation, calcium carbonate biomineralization

Product: electron transfer flavoprotein (alpha subunit)

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Electron transport and ATP synthesis → Electron transport/ other
• Metabolism → Lipid metabolism → Utilization of lipids → Utilization of fatty acids

Gene

Coordinates: 2,915,365 2,916,342

The protein

Protein family

• ETF alpha-subunit/fixB family (according to Swiss-Prot)

Structure

• 1EFV (the human protein, 39% identity) PubMed

Expression and Regulation

Operons

• Genes: lcfA-fadR-fadB-etfB-etfA
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • FadR: repression, PubMed, in FadR regulon
  • CcpA: repression, PubMed, in CcpA regulon

  Regulation

  • induced by long chain acyl-CoA (C14 ... C20) (FadR) PubMed
  • expression of the operon is strongly induced during biofilm formation PubMed

• Genes: fadR-fadB-etfB-etfA
  Description: PubMed

  Regulation

  • induced by long-chain fatty acids (FadR) PubMed

Biological materials

Mutant

• 1A856 ( etfA::cat), PubMed, available at BGSC
• BKE28520 (etfA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCTAACATCCCCCTGTT, downstream forward: _UP4_TAAAGGAAATCCCGGACTTT
• BKK28520 (etfA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCTAACATCCCCCTGTT, downstream forward: _UP4_TAAAGGAAATCCCGGACTTT

References

Reviews

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Original Publications

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